Let's face it, when dealing with segmentation of microscopy data we often do not have time to check that everything is correct, because it is a tedious and very time consuming process. Cell-ACDC comes to the rescue! We combined the currently best available neural network models (such as YeaZ, cellpose, StarDist, YeastMate, omnipose, delta, etc.) and we complemented them with a fast and intuitive GUI.

We developed and implemented several smart functionalities such as real-time continuous tracking, automatic propagation of error correction, and several tools to facilitate manual correction, from simple yet useful brush and eraser to more complex flood fill (magic wand) and Random Walker segmentation routines.

You can find Cell-ACDC on GitHub. Do not hesitate to contact us if you have questions!

Padovani, F., Mairhörmann, B., Falter-Braun, P., Lengefeld, J. & Schmoller, K.M. (2022) Segmentation, tracking and cell cycle analysis of live-cell imaging data with Cell-ACDC, BMC Biology, 20, 1-18